Construct: shRNA TRCN0000316975

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133347.1-3413s21c1
DNA Barcode:
GCTGCATAAGTCAACCATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dhx30 (72831)
Gene Description:
DEAH (Asp-Glu-Ala-His) box polypeptide 30
Transcript:
RefSeq NM_133347.1 (NON-CURRENT)
Match location:
Position 3413 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316975)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCATAAGTCAACCATTAA
Hairpin Sequence:
5'-CCGG-GCTGCATAAGTCAACCATTAA-CTCGAG-TTAATGGTTGACTTATGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCATAAGTCAACCATTAACTCGAGTTAATGGTTGACTTATGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCATAAGTCAACCATTAACTCGAGTTAATGGTTGACTTATGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000113045