Construct: shRNA TRCN0000317029

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021542.4-1803s21c1
DNA Barcode:
CGAATACAACAAGGCAGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnk5 (16529)
Gene Description:
potassium channel, subfamily K, member 5
Transcript:
RefSeq NM_021542.4 (CURRENT)
Match location:
Position 1803 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317029)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAATACAACAAGGCAGATAA
Hairpin Sequence:
5'-CCGG-CGAATACAACAAGGCAGATAA-CTCGAG-TTATCTGCCTTGTTGTATTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAATACAACAAGGCAGATAACTCGAGTTATCTGCCTTGTTGTATTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAATACAACAAGGCAGATAACTCGAGTTATCTGCCTTGTTGTATTCG-3'

Other clones with same target sequence:

  1. TRCN0000070011