Construct: shRNA TRCN0000317195

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134068.2-112s21c1
DNA Barcode:
AGGAACAAGGTGACACACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dusp22 (105352)
Gene Description:
dual specificity phosphatase 22
Transcript:
RefSeq NM_134068.2 (NON-CURRENT)
Match location:
Position 112 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317195)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAACAAGGTGACACACATT
Hairpin Sequence:
5'-CCGG-AGGAACAAGGTGACACACATT-CTCGAG-AATGTGTGTCACCTTGTTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAACAAGGTGACACACATTCTCGAGAATGTGTGTCACCTTGTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAACAAGGTGACACACATTCTCGAGAATGTGTGTCACCTTGTTCCT-3'

Other clones with same target sequence:

  1. TRCN0000081347