Construct: shRNA TRCN0000317222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010743.2-677s21c1
DNA Barcode:
GCTGCAATATCCCTGATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Il1rl1 (17082)
Gene Description:
interleukin 1 receptor-like 1
Transcript:
RefSeq NM_010743.2 (NON-CURRENT)
Match location:
Position 677 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCAATATCCCTGATTATT
Hairpin Sequence:
5'-CCGG-GCTGCAATATCCCTGATTATT-CTCGAG-AATAATCAGGGATATTGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCAATATCCCTGATTATTCTCGAGAATAATCAGGGATATTGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCAATATCCCTGATTATTCTCGAGAATAATCAGGGATATTGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000039057