Construct: shRNA TRCN0000317274

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053103.4-2620s21c1
DNA Barcode:
CGCCTTTGAAATGGATGACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Entpd7 (93685)
Gene Description:
ectonucleoside triphosphate diphosphohydrolase 7
Transcript:
RefSeq NM_053103.4 (NON-CURRENT)
Match location:
Position 2620 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317274)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTTTGAAATGGATGACTT
Hairpin Sequence:
5'-CCGG-CGCCTTTGAAATGGATGACTT-CTCGAG-AAGTCATCCATTTCAAAGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTTTGAAATGGATGACTTCTCGAGAAGTCATCCATTTCAAAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTTTGAAATGGATGACTTCTCGAGAAGTCATCCATTTCAAAGGCG-3'

Other clones with same target sequence:

  1. TRCN0000081303