Construct: shRNA TRCN0000317288

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025736.2-367s21c1
DNA Barcode:
GACGATGCTATACAACTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Emc2 (66736)
Gene Description:
ER membrane protein complex subunit 2
Transcript:
RefSeq NM_025736.2 (CURRENT)
Match location:
Position 367 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317288)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGATGCTATACAACTGTAT
Hairpin Sequence:
5'-CCGG-GACGATGCTATACAACTGTAT-CTCGAG-ATACAGTTGTATAGCATCGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGATGCTATACAACTGTATCTCGAGATACAGTTGTATAGCATCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGATGCTATACAACTGTATCTCGAGATACAGTTGTATAGCATCGTC-3'

Other clones with same target sequence:

  1. TRCN0000125568