Construct: shRNA TRCN0000317345

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008991.2-498s21c1
DNA Barcode:
CTCTTATCTCTCTGGTTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcd3 (19299)
Gene Description:
ATP-binding cassette, sub-family D (ALD), member 3
Transcript:
RefSeq NM_008991.2 (CURRENT)
Match location:
Position 498 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317345)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTTATCTCTCTGGTTAATA
Hairpin Sequence:
5'-CCGG-CTCTTATCTCTCTGGTTAATA-CTCGAG-TATTAACCAGAGAGATAAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTTATCTCTCTGGTTAATACTCGAGTATTAACCAGAGAGATAAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTTATCTCTCTGGTTAATACTCGAGTATTAACCAGAGAGATAAGAG-3'

Other clones with same target sequence:

  1. TRCN0000105339