Construct: shRNA TRCN0000317410

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153319.2-318s21c1
DNA Barcode:
CCAGGAACTCTCAACCTCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Amot (27494)
Gene Description:
angiomotin
Transcript:
RefSeq NM_153319.2 (NON-CURRENT)
Match location:
Position 318 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317410)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAACTCTCAACCTCATA
Hairpin Sequence:
5'-CCGG-CCAGGAACTCTCAACCTCATA-CTCGAG-TATGAGGTTGAGAGTTCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAACTCTCAACCTCATACTCGAGTATGAGGTTGAGAGTTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAACTCTCAACCTCATACTCGAGTATGAGGTTGAGAGTTCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000126880