Construct: shRNA TRCN0000317534

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019994.4-3267s21c1
DNA Barcode:
CCAGCCTAACTACGCTTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ralgapa1 (56784)
Gene Description:
Ral GTPase activating protein, alpha subunit 1
Transcript:
RefSeq NM_019994.4 (NON-CURRENT)
Match location:
Position 3267 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317534)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCCTAACTACGCTTCATT
Hairpin Sequence:
5'-CCGG-CCAGCCTAACTACGCTTCATT-CTCGAG-AATGAAGCGTAGTTAGGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCCTAACTACGCTTCATTCTCGAGAATGAAGCGTAGTTAGGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCCTAACTACGCTTCATTCTCGAGAATGAAGCGTAGTTAGGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000088159