Construct: shRNA TRCN0000317613

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024199.2-1042s21c1
DNA Barcode:
GTAGCGAAACTCTGGGAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cstf1 (67337)
Gene Description:
cleavage stimulation factor, 3' pre-RNA, subunit 1
Transcript:
RefSeq NM_024199.2 (CURRENT)
Match location:
Position 1042 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317613)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGCGAAACTCTGGGAGATT
Hairpin Sequence:
5'-CCGG-GTAGCGAAACTCTGGGAGATT-CTCGAG-AATCTCCCAGAGTTTCGCTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGCGAAACTCTGGGAGATTCTCGAGAATCTCCCAGAGTTTCGCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGCGAAACTCTGGGAGATTCTCGAGAATCTCCCAGAGTTTCGCTAC-3'

Other clones with same target sequence:

  1. TRCN0000112011