Construct: shRNA TRCN0000317617

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133887.4-867s21c1
DNA Barcode:
GTCAGTGATTGTTACAGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stx12 (100226)
Gene Description:
syntaxin 12
Transcript:
RefSeq NM_133887.4 (CURRENT)
Match location:
Position 867 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317617)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGTGATTGTTACAGTCTT
Hairpin Sequence:
5'-CCGG-GTCAGTGATTGTTACAGTCTT-CTCGAG-AAGACTGTAACAATCACTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGTGATTGTTACAGTCTTCTCGAGAAGACTGTAACAATCACTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGTGATTGTTACAGTCTTCTCGAGAAGACTGTAACAATCACTGAC-3'

Other clones with same target sequence:

  1. TRCN0000100933