Construct: shRNA TRCN0000317719

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010787.1-192s21c1
DNA Barcode:
AGCAGTGAAGAACCCGAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mea1 (17256)
Gene Description:
male enhanced antigen 1
Transcript:
RefSeq NM_010787.1 (NON-CURRENT)
Match location:
Position 192 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317719)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGTGAAGAACCCGAGGAA
Hairpin Sequence:
5'-CCGG-AGCAGTGAAGAACCCGAGGAA-CTCGAG-TTCCTCGGGTTCTTCACTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGTGAAGAACCCGAGGAACTCGAGTTCCTCGGGTTCTTCACTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGTGAAGAACCCGAGGAACTCGAGTTCCTCGGGTTCTTCACTGCT-3'

Other clones with same target sequence:

  1. TRCN0000106399