Construct: shRNA TRCN0000317728

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029090.3-501s21c1
DNA Barcode:
GTGGTGAAAGAGTTCAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Naa60 (74763)
Gene Description:
N(alpha)-acetyltransferase 60, NatF catalytic subunit
Transcript:
RefSeq NM_029090.3 (CURRENT)
Match location:
Position 501 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317728)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGTGAAAGAGTTCAGGAAA
Hairpin Sequence:
5'-CCGG-GTGGTGAAAGAGTTCAGGAAA-CTCGAG-TTTCCTGAACTCTTTCACCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGTGAAAGAGTTCAGGAAACTCGAGTTTCCTGAACTCTTTCACCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGTGAAAGAGTTCAGGAAACTCGAGTTTCCTGAACTCTTTCACCAC-3'

Other clones with same target sequence:

  1. TRCN0000114644