Construct: shRNA TRCN0000317757

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026542.2-306s21c1
DNA Barcode:
CCCAGGGAAGTGCCTCCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc25a39 (68066)
Gene Description:
solute carrier family 25, member 39
Transcript:
RefSeq NM_026542.2 (NON-CURRENT)
Match location:
Position 306 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317757)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGGAAGTGCCTCCTATA
Hairpin Sequence:
5'-CCGG-CCCAGGGAAGTGCCTCCTATA-CTCGAG-TATAGGAGGCACTTCCCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGGAAGTGCCTCCTATACTCGAGTATAGGAGGCACTTCCCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGGAAGTGCCTCCTATACTCGAGTATAGGAGGCACTTCCCTGGG-3'

Other clones with same target sequence:

  1. TRCN0000114278