Construct: shRNA TRCN0000317777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018813.2-735s21c1
DNA Barcode:
GCGAGAAGCAAGATTCTGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpsf3 (54451)
Gene Description:
cleavage and polyadenylation specificity factor 3
Transcript:
RefSeq NM_018813.2 (NON-CURRENT)
Match location:
Position 735 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGAAGCAAGATTCTGCAA
Hairpin Sequence:
5'-CCGG-GCGAGAAGCAAGATTCTGCAA-CTCGAG-TTGCAGAATCTTGCTTCTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGAAGCAAGATTCTGCAACTCGAGTTGCAGAATCTTGCTTCTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGAAGCAAGATTCTGCAACTCGAGTTGCAGAATCTTGCTTCTCGC-3'

Other clones with same target sequence:

  1. TRCN0000119575