Construct: shRNA TRCN0000317823

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134255.2-389s21c1
DNA Barcode:
GCTGTCTCTCTACATGTTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Elovl5 (68801)
Gene Description:
ELOVL family member 5, elongation of long chain fatty acids (yeast)
Transcript:
RefSeq NM_134255.2 (NON-CURRENT)
Match location:
Position 389 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317823)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCTCTCTACATGTTCTA
Hairpin Sequence:
5'-CCGG-GCTGTCTCTCTACATGTTCTA-CTCGAG-TAGAACATGTAGAGAGACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCTCTCTACATGTTCTACTCGAGTAGAACATGTAGAGAGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCTCTCTACATGTTCTACTCGAGTAGAACATGTAGAGAGACAGC-3'

Other clones with same target sequence:

  1. TRCN0000126312