Construct: shRNA TRCN0000317870

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178688.3-1999s21c1
DNA Barcode:
CCCAACGAGATACCAAAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ablim1 (226251)
Gene Description:
actin-binding LIM protein 1
Transcript:
RefSeq NM_178688.3 (CURRENT)
Match location:
Position 1999 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317870)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACGAGATACCAAAGATT
Hairpin Sequence:
5'-CCGG-CCCAACGAGATACCAAAGATT-CTCGAG-AATCTTTGGTATCTCGTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACGAGATACCAAAGATTCTCGAGAATCTTTGGTATCTCGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACGAGATACCAAAGATTCTCGAGAATCTTTGGTATCTCGTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000099293