Construct: shRNA TRCN0000317879

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009456.2-224s21c1
DNA Barcode:
CCACCCAAGATCACATTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ube2l3 (22195)
Gene Description:
ubiquitin-conjugating enzyme E2L 3
Transcript:
RefSeq NM_009456.2 (CURRENT)
Match location:
Position 224 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317879)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCCAAGATCACATTTAAA
Hairpin Sequence:
5'-CCGG-CCACCCAAGATCACATTTAAA-CTCGAG-TTTAAATGTGATCTTGGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCCAAGATCACATTTAAACTCGAGTTTAAATGTGATCTTGGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCCAAGATCACATTTAAACTCGAGTTTAAATGTGATCTTGGGTGG-3'

Other clones with same target sequence:

  1. TRCN0000040827