Construct: shRNA TRCN0000317931
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_145542.3-802s21c1
- DNA Barcode:
- CAACATCTATTCAACTCAGAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Ahcyl1 (229709)
- Gene Description:
- S-adenosylhomocysteine hydrolase-like 1
- Transcript:
- RefSeq NM_145542.3 (CURRENT)
- Match location:
- Position 802 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000317931)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAACATCTATTCAACTCAGAA
- Hairpin Sequence:
-
5'-CCGG-CAACATCTATTCAACTCAGAA-CTCGAG-TTCTGAGTTGAATAGATGTTG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAACATCTATTCAACTCAGAACTCGAGTTCTGAGTTGAATAGATGTTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAACATCTATTCAACTCAGAACTCGAGTTCTGAGTTGAATAGATGTTG-3'
Other clones with same target sequence:
-
TRCN0000101598
