Construct: shRNA TRCN0000317931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145542.3-802s21c1
DNA Barcode:
CAACATCTATTCAACTCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ahcyl1 (229709)
Gene Description:
S-adenosylhomocysteine hydrolase-like 1
Transcript:
RefSeq NM_145542.3 (CURRENT)
Match location:
Position 802 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACATCTATTCAACTCAGAA
Hairpin Sequence:
5'-CCGG-CAACATCTATTCAACTCAGAA-CTCGAG-TTCTGAGTTGAATAGATGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACATCTATTCAACTCAGAACTCGAGTTCTGAGTTGAATAGATGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACATCTATTCAACTCAGAACTCGAGTTCTGAGTTGAATAGATGTTG-3'

Other clones with same target sequence:

  1. TRCN0000101598