Construct: shRNA TRCN0000317939

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011308.2-7774s21c1
DNA Barcode:
CCTCTAATACAGGCACTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ncor1 (20185)
Gene Description:
nuclear receptor co-repressor 1
Transcript:
RefSeq NM_011308.2 (NON-CURRENT)
Match location:
Position 7774 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317939)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTAATACAGGCACTTCAA
Hairpin Sequence:
5'-CCGG-CCTCTAATACAGGCACTTCAA-CTCGAG-TTGAAGTGCCTGTATTAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTAATACAGGCACTTCAACTCGAGTTGAAGTGCCTGTATTAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTAATACAGGCACTTCAACTCGAGTTGAAGTGCCTGTATTAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000096474