Construct: shRNA TRCN0000317947

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030732.3-827s21c1
DNA Barcode:
GCACATACCATCGCAAATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbl1xr1 (81004)
Gene Description:
transducin (beta)-like 1X-linked receptor 1
Transcript:
RefSeq NM_030732.3 (CURRENT)
Match location:
Position 827 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000317947)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATACCATCGCAAATAAT
Hairpin Sequence:
5'-CCGG-GCACATACCATCGCAAATAAT-CTCGAG-ATTATTTGCGATGGTATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATACCATCGCAAATAATCTCGAGATTATTTGCGATGGTATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATACCATCGCAAATAATCTCGAGATTATTTGCGATGGTATGTGC-3'

Other clones with same target sequence:

  1. TRCN0000109344