Construct: shRNA TRCN0000318070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008503.4-285s21c1
DNA Barcode:
GCCCATTAAGGAGTCTGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rps2 (16898)
Gene Description:
ribosomal protein S2
Transcript:
RefSeq NM_008503.4 (NON-CURRENT)
Match location:
Position 285 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318070)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATTAAGGAGTCTGAGAT
Hairpin Sequence:
5'-CCGG-GCCCATTAAGGAGTCTGAGAT-CTCGAG-ATCTCAGACTCCTTAATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATTAAGGAGTCTGAGATCTCGAGATCTCAGACTCCTTAATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATTAAGGAGTCTGAGATCTCGAGATCTCAGACTCCTTAATGGGC-3'

Other clones with same target sequence:

  1. TRCN0000054675