Construct: shRNA TRCN0000318136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_054077.3-1021s21c1
DNA Barcode:
GTCTCACAACAAGATCAGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prelp (116847)
Gene Description:
proline arginine-rich end leucine-rich repeat
Transcript:
RefSeq NM_054077.3 (NON-CURRENT)
Match location:
Position 1021 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTCACAACAAGATCAGCAA
Hairpin Sequence:
5'-CCGG-GTCTCACAACAAGATCAGCAA-CTCGAG-TTGCTGATCTTGTTGTGAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTCACAACAAGATCAGCAACTCGAGTTGCTGATCTTGTTGTGAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTCACAACAAGATCAGCAACTCGAGTTGCTGATCTTGTTGTGAGAC-3'

Other clones with same target sequence:

  1. TRCN0000094462