Construct: shRNA TRCN0000318212

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139272.2-547s21c1
DNA Barcode:
CGTGGTGATCACATTCCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Galnt2 (108148)
Gene Description:
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2
Transcript:
RefSeq NM_139272.2 (CURRENT)
Match location:
Position 547 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318212)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGTGATCACATTCCACAA
Hairpin Sequence:
5'-CCGG-CGTGGTGATCACATTCCACAA-CTCGAG-TTGTGGAATGTGATCACCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGTGATCACATTCCACAACTCGAGTTGTGGAATGTGATCACCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGTGATCACATTCCACAACTCGAGTTGTGGAATGTGATCACCACG-3'

Other clones with same target sequence:

  1. TRCN0000110268