Construct: shRNA TRCN0000318288

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201245.3-3728s21c1
DNA Barcode:
GCACACTCACTGTCAGTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mprip (26936)
Gene Description:
myosin phosphatase Rho interacting protein
Transcript:
RefSeq NM_201245.3 (CURRENT)
Match location:
Position 3728 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318288)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACTCACTGTCAGTATTA
Hairpin Sequence:
5'-CCGG-GCACACTCACTGTCAGTATTA-CTCGAG-TAATACTGACAGTGAGTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACTCACTGTCAGTATTACTCGAGTAATACTGACAGTGAGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACTCACTGTCAGTATTACTCGAGTAATACTGACAGTGAGTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000091173