Construct: shRNA TRCN0000318375

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006908.4-340s21c1
DNA Barcode:
CCCTACTGTCTTTGACAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAC1 (5879)
Gene Description:
Rac family small GTPase 1
Transcript:
RefSeq NM_006908.4 (NON-CURRENT)
Match location:
Position 340 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318375)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTACTGTCTTTGACAATTA
Hairpin Sequence:
5'-CCGG-CCCTACTGTCTTTGACAATTA-CTCGAG-TAATTGTCAAAGACAGTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTACTGTCTTTGACAATTACTCGAGTAATTGTCAAAGACAGTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTACTGTCTTTGACAATTACTCGAGTAATTGTCAAAGACAGTAGGG-3'

Other clones with same target sequence:

  1. TRCN0000004873