Construct: shRNA TRCN0000318485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002867.2-618s21c1
DNA Barcode:
AGTGCAAAGGAGAACATCAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB3B (5865)
Gene Description:
RAB3B, member RAS oncogene family
Transcript:
RefSeq NM_002867.2 (NON-CURRENT)
Match location:
Position 618 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGCAAAGGAGAACATCAGT
Hairpin Sequence:
5'-CCGG-AGTGCAAAGGAGAACATCAGT-CTCGAG-ACTGATGTTCTCCTTTGCACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGCAAAGGAGAACATCAGTCTCGAGACTGATGTTCTCCTTTGCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGCAAAGGAGAACATCAGTCTCGAGACTGATGTTCTCCTTTGCACT-3'

Other clones with same target sequence:

  1. TRCN0000000216