Construct: shRNA TRCN0000318531

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005813.3-3393s21c1
DNA Barcode:
CGGGAAACTGAATAATAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKD3 (23683)
Gene Description:
protein kinase D3
Transcript:
RefSeq NM_005813.3 (NON-CURRENT)
Match location:
Position 3393 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318531)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAAACTGAATAATAAGAA
Hairpin Sequence:
5'-CCGG-CGGGAAACTGAATAATAAGAA-CTCGAG-TTCTTATTATTCAGTTTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAAACTGAATAATAAGAACTCGAGTTCTTATTATTCAGTTTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAAACTGAATAATAAGAACTCGAGTTCTTATTATTCAGTTTCCCG-3'

Other clones with same target sequence:

  1. TRCN0000001412