Construct: shRNA TRCN0000318551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004156.2-593s21c1
DNA Barcode:
AGCCGACAAATTACCCAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP2CB (5516)
Gene Description:
protein phosphatase 2 catalytic subunit beta
Transcript:
RefSeq NM_004156.2 (NON-CURRENT)
Match location:
Position 593 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCGACAAATTACCCAAGTA
Hairpin Sequence:
5'-CCGG-AGCCGACAAATTACCCAAGTA-CTCGAG-TACTTGGGTAATTTGTCGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCGACAAATTACCCAAGTACTCGAGTACTTGGGTAATTTGTCGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCGACAAATTACCCAAGTACTCGAGTACTTGGGTAATTTGTCGGCT-3'

Other clones with same target sequence:

  1. TRCN0000002506