Construct: shRNA TRCN0000318553
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_000212.2-2347s21c1
- DNA Barcode:
- CCACGTCTACCTTCACCAATA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITGB3 (3690)
- Gene Description:
- integrin subunit beta 3
- Transcript:
- RefSeq NM_000212.2 (CURRENT)
- Match location:
- Position 2347 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000318553)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCACGTCTACCTTCACCAATA
- Hairpin Sequence:
-
5'-CCGG-CCACGTCTACCTTCACCAATA-CTCGAG-TATTGGTGAAGGTAGACGTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCACGTCTACCTTCACCAATACTCGAGTATTGGTGAAGGTAGACGTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCACGTCTACCTTCACCAATACTCGAGTATTGGTGAAGGTAGACGTGG-3'
Other clones with same target sequence:
-
TRCN0000003235
