Construct: shRNA TRCN0000318590

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005813.3-3181s21c1
DNA Barcode:
CACTTCATTATGGCTCCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKD3 (23683)
Gene Description:
protein kinase D3
Transcript:
RefSeq NM_005813.3 (NON-CURRENT)
Match location:
Position 3181 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318590)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTTCATTATGGCTCCTAAT
Hairpin Sequence:
5'-CCGG-CACTTCATTATGGCTCCTAAT-CTCGAG-ATTAGGAGCCATAATGAAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTTCATTATGGCTCCTAATCTCGAGATTAGGAGCCATAATGAAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTTCATTATGGCTCCTAATCTCGAGATTAGGAGCCATAATGAAGTG-3'

Other clones with same target sequence:

  1. TRCN0000195275