Construct: shRNA TRCN0000318689

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001896.2-410s21c1
DNA Barcode:
CGTGGTGGAACAAATATCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK2A2 (1459)
Gene Description:
casein kinase 2 alpha 2
Transcript:
RefSeq NM_001896.2 (NON-CURRENT)
Match location:
Position 410 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318689)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGTGGAACAAATATCATT
Hairpin Sequence:
5'-CCGG-CGTGGTGGAACAAATATCATT-CTCGAG-AATGATATTTGTTCCACCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGTGGAACAAATATCATTCTCGAGAATGATATTTGTTCCACCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGTGGAACAAATATCATTCTCGAGAATGATATTTGTTCCACCACG-3'

Other clones with same target sequence:

  1. TRCN0000000613