Construct: shRNA TRCN0000318694

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014602.2-4813s21c1
DNA Barcode:
GTTAAGATGTACTTGACTAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3R4 (30849)
Gene Description:
phosphoinositide-3-kinase regulatory subunit 4
Transcript:
RefSeq NM_014602.2 (NON-CURRENT)
Match location:
Position 4813 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318694)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTAAGATGTACTTGACTAGT
Hairpin Sequence:
5'-CCGG-GTTAAGATGTACTTGACTAGT-CTCGAG-ACTAGTCAAGTACATCTTAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTAAGATGTACTTGACTAGTCTCGAGACTAGTCAAGTACATCTTAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTAAGATGTACTTGACTAGTCTCGAGACTAGTCAAGTACATCTTAAC-3'

Other clones with same target sequence:

  1. TRCN0000196467