Construct: shRNA TRCN0000318827

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004087.2-1001s21c1
DNA Barcode:
CGGGTCAATGACTGTATATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DLG1 (1739)
Gene Description:
discs large MAGUK scaffold protein 1
Transcript:
RefSeq NM_004087.2 (CURRENT)
Match location:
Position 1001 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318827)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGTCAATGACTGTATATTA
Hairpin Sequence:
5'-CCGG-CGGGTCAATGACTGTATATTA-CTCGAG-TAATATACAGTCATTGACCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGTCAATGACTGTATATTACTCGAGTAATATACAGTCATTGACCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGTCAATGACTGTATATTACTCGAGTAATATACAGTCATTGACCCG-3'

Other clones with same target sequence:

  1. TRCN0000006101