Construct: shRNA TRCN0000318838

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017672.3-3256s21c1
DNA Barcode:
GCCAATATGTTCTACATTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPM7 (54822)
Gene Description:
transient receptor potential cation channel subfamily M member 7
Transcript:
RefSeq NM_017672.3 (NON-CURRENT)
Match location:
Position 3256 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318838)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATATGTTCTACATTGTA
Hairpin Sequence:
5'-CCGG-GCCAATATGTTCTACATTGTA-CTCGAG-TACAATGTAGAACATATTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATATGTTCTACATTGTACTCGAGTACAATGTAGAACATATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATATGTTCTACATTGTACTCGAGTACAATGTAGAACATATTGGC-3'

Other clones with same target sequence:

  1. TRCN0000021561