Construct: shRNA TRCN0000318868

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017983.5-334s21c1
DNA Barcode:
GCAGATGAACGTGTATCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WIPI1 (55062)
Gene Description:
WD repeat domain, phosphoinositide interacting 1
Transcript:
RefSeq NM_017983.5 (NON-CURRENT)
Match location:
Position 334 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318868)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATGAACGTGTATCACTT
Hairpin Sequence:
5'-CCGG-GCAGATGAACGTGTATCACTT-CTCGAG-AAGTGATACACGTTCATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATGAACGTGTATCACTTCTCGAGAAGTGATACACGTTCATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATGAACGTGTATCACTTCTCGAGAAGTGATACACGTTCATCTGC-3'

Other clones with same target sequence:

  1. TRCN0000154084