Construct: shRNA TRCN0000318995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024910.1-518s21c1
DNA Barcode:
CCAGATTGAACAAGGGAAGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF767P (79970)
Gene Description:
zinc finger family member 767, pseudogene
Transcript:
RefSeq NM_024910.1 (NON-CURRENT)
Match location:
Position 518 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000318995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATTGAACAAGGGAAGGA
Hairpin Sequence:
5'-CCGG-CCAGATTGAACAAGGGAAGGA-CTCGAG-TCCTTCCCTTGTTCAATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATTGAACAAGGGAAGGACTCGAGTCCTTCCCTTGTTCAATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATTGAACAAGGGAAGGACTCGAGTCCTTCCCTTGTTCAATCTGG-3'

Other clones with same target sequence:

  1. TRCN0000166493