Construct: shRNA TRCN0000319005

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004898.2-932s21c1
DNA Barcode:
GCGAGGAACAATAGACCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLOCK (9575)
Gene Description:
clock circadian regulator
Transcript:
RefSeq NM_004898.2 (NON-CURRENT)
Match location:
Position 932 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319005)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGGAACAATAGACCCAAA
Hairpin Sequence:
5'-CCGG-GCGAGGAACAATAGACCCAAA-CTCGAG-TTTGGGTCTATTGTTCCTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGGAACAATAGACCCAAACTCGAGTTTGGGTCTATTGTTCCTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGGAACAATAGACCCAAACTCGAGTTTGGGTCTATTGTTCCTCGC-3'

Other clones with same target sequence:

  1. TRCN0000018977