Construct: shRNA TRCN0000319015

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004763.3-212s21c1
DNA Barcode:
GCAGTTCCCAAAGTAGCGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITGB1BP1 (9270)
Gene Description:
integrin subunit beta 1 binding protein 1
Transcript:
RefSeq NM_004763.3 (NON-CURRENT)
Match location:
Position 212 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319015)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTCCCAAAGTAGCGAAA
Hairpin Sequence:
5'-CCGG-GCAGTTCCCAAAGTAGCGAAA-CTCGAG-TTTCGCTACTTTGGGAACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTCCCAAAGTAGCGAAACTCGAGTTTCGCTACTTTGGGAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTCCCAAAGTAGCGAAACTCGAGTTTCGCTACTTTGGGAACTGC-3'

Other clones with same target sequence:

  1. TRCN0000122920