Construct: shRNA TRCN0000319152

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004459.6-1709s21c1
DNA Barcode:
GCGGCAGCTAATGAAGAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BPTF (2186)
Gene Description:
bromodomain PHD finger transcription factor
Transcript:
RefSeq NM_004459.6 (NON-CURRENT)
Match location:
Position 1709 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319152)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCAGCTAATGAAGAAATT
Hairpin Sequence:
5'-CCGG-GCGGCAGCTAATGAAGAAATT-CTCGAG-AATTTCTTCATTAGCTGCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCAGCTAATGAAGAAATTCTCGAGAATTTCTTCATTAGCTGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCAGCTAATGAAGAAATTCTCGAGAATTTCTTCATTAGCTGCCGC-3'

Other clones with same target sequence:

  1. TRCN0000016819