Construct: shRNA TRCN0000319262

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015050.2-3229s21c1
DNA Barcode:
GCCAGGAGAACCTGCTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMTR1 (23070)
Gene Description:
cap methyltransferase 1
Transcript:
RefSeq NM_015050.2 (NON-CURRENT)
Match location:
Position 3229 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319262)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGAGAACCTGCTATAAA
Hairpin Sequence:
5'-CCGG-GCCAGGAGAACCTGCTATAAA-CTCGAG-TTTATAGCAGGTTCTCCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGAGAACCTGCTATAAACTCGAGTTTATAGCAGGTTCTCCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGAGAACCTGCTATAAACTCGAGTTTATAGCAGGTTCTCCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000142265