Construct: shRNA TRCN0000319279

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001695.4-316s21c1
DNA Barcode:
CGGCAACTTCAAAGAACAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V1C1 (528)
Gene Description:
ATPase H+ transporting V1 subunit C1
Transcript:
RefSeq NM_001695.4 (NON-CURRENT)
Match location:
Position 316 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319279)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAACTTCAAAGAACAATA
Hairpin Sequence:
5'-CCGG-CGGCAACTTCAAAGAACAATA-CTCGAG-TATTGTTCTTTGAAGTTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAACTTCAAAGAACAATACTCGAGTATTGTTCTTTGAAGTTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAACTTCAAAGAACAATACTCGAGTATTGTTCTTTGAAGTTGCCG-3'

Other clones with same target sequence:

  1. TRCN0000029567