Construct: shRNA TRCN0000319310
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_006082.2-1053s21c1
- DNA Barcode:
- GCTGAGAAAGCCTACCATGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TUBA1B (10376)
- Gene Description:
- tubulin alpha 1b
- Transcript:
- RefSeq NM_006082.2 (NON-CURRENT)
- Match location:
- Position 1053 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000319310)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTGAGAAAGCCTACCATGAA
- Hairpin Sequence:
-
5'-CCGG-GCTGAGAAAGCCTACCATGAA-CTCGAG-TTCATGGTAGGCTTTCTCAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTGAGAAAGCCTACCATGAACTCGAGTTCATGGTAGGCTTTCTCAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTGAGAAAGCCTACCATGAACTCGAGTTCATGGTAGGCTTTCTCAGC-3'
Other clones with same target sequence:
-
TRCN0000157518
