Construct: shRNA TRCN0000319318

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004844.3-778s21c1
DNA Barcode:
CTGGAGAAGAAACTCAAGAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3BP5 (9467)
Gene Description:
SH3 domain binding protein 5
Transcript:
RefSeq NM_004844.3 (NON-CURRENT)
Match location:
Position 778 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319318)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGAGAAGAAACTCAAGAGA
Hairpin Sequence:
5'-CCGG-CTGGAGAAGAAACTCAAGAGA-CTCGAG-TCTCTTGAGTTTCTTCTCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGAGAAGAAACTCAAGAGACTCGAGTCTCTTGAGTTTCTTCTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGAGAAGAAACTCAAGAGACTCGAGTCTCTTGAGTTTCTTCTCCAG-3'

Other clones with same target sequence:

  1. TRCN0000142779