Construct: shRNA TRCN0000319330

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018590.3-864s21c1
DNA Barcode:
CGAGATGAATTGGTGGAAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSGALNACT2 (55454)
Gene Description:
chondroitin sulfate N-acetylgalactosaminyltransferase 2
Transcript:
RefSeq NM_018590.3 (NON-CURRENT)
Match location:
Position 864 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319330)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGATGAATTGGTGGAAGTT
Hairpin Sequence:
5'-CCGG-CGAGATGAATTGGTGGAAGTT-CTCGAG-AACTTCCACCAATTCATCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGATGAATTGGTGGAAGTTCTCGAGAACTTCCACCAATTCATCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGATGAATTGGTGGAAGTTCTCGAGAACTTCCACCAATTCATCTCG-3'

Other clones with same target sequence:

  1. TRCN0000152477