Construct: shRNA TRCN0000319345

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003822.3-1616s21c1
DNA Barcode:
CCTCTACTACAAGCACCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NR5A2 (2494)
Gene Description:
nuclear receptor subfamily 5 group A member 2
Transcript:
RefSeq NM_003822.3 (NON-CURRENT)
Match location:
Position 1616 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319345)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTACTACAAGCACCTGAA
Hairpin Sequence:
5'-CCGG-CCTCTACTACAAGCACCTGAA-CTCGAG-TTCAGGTGCTTGTAGTAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTACTACAAGCACCTGAACTCGAGTTCAGGTGCTTGTAGTAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTACTACAAGCACCTGAACTCGAGTTCAGGTGCTTGTAGTAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000019658