Construct: shRNA TRCN0000319391

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014043.3-357s21c1
DNA Barcode:
GCAGCTTTAGAGAAACAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHMP2B (25978)
Gene Description:
charged multivesicular body protein 2B
Transcript:
RefSeq NM_014043.3 (NON-CURRENT)
Match location:
Position 357 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319391)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTTTAGAGAAACAAGAA
Hairpin Sequence:
5'-CCGG-GCAGCTTTAGAGAAACAAGAA-CTCGAG-TTCTTGTTTCTCTAAAGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTTTAGAGAAACAAGAACTCGAGTTCTTGTTTCTCTAAAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTTTAGAGAAACAAGAACTCGAGTTCTTGTTTCTCTAAAGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000129922