Construct: shRNA TRCN0000319397

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004844.3-325s21c1
DNA Barcode:
CAGGGAGAACTGGAGAAGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3BP5 (9467)
Gene Description:
SH3 domain binding protein 5
Transcript:
RefSeq NM_004844.3 (NON-CURRENT)
Match location:
Position 325 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319397)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGGAGAACTGGAGAAGTTA
Hairpin Sequence:
5'-CCGG-CAGGGAGAACTGGAGAAGTTA-CTCGAG-TAACTTCTCCAGTTCTCCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGGAGAACTGGAGAAGTTACTCGAGTAACTTCTCCAGTTCTCCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGGAGAACTGGAGAAGTTACTCGAGTAACTTCTCCAGTTCTCCCTG-3'

Other clones with same target sequence:

  1. TRCN0000139445