Construct: shRNA TRCN0000319412

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144516.2-2263s21c1
DNA Barcode:
CTGGCGGCATGTGATTGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zmynd11 (66505)
Gene Description:
zinc finger, MYND domain containing 11
Transcript:
RefSeq NM_144516.2 (NON-CURRENT)
Match location:
Position 2263 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319412)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGCGGCATGTGATTGAATA
Hairpin Sequence:
5'-CCGG-CTGGCGGCATGTGATTGAATA-CTCGAG-TATTCAATCACATGCCGCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGCGGCATGTGATTGAATACTCGAGTATTCAATCACATGCCGCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGCGGCATGTGATTGAATACTCGAGTATTCAATCACATGCCGCCAG-3'

Other clones with same target sequence:

(none)