Construct: shRNA TRCN0000319473

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201244.1-148s21c1
DNA Barcode:
CCAAGAGGCTCGGGTCTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Copg1 (54161)
Gene Description:
coatomer protein complex, subunit gamma 1
Transcript:
RefSeq NM_201244.1 (CURRENT)
Match location:
Position 148 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319473)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGAGGCTCGGGTCTTTAA
Hairpin Sequence:
5'-CCGG-CCAAGAGGCTCGGGTCTTTAA-CTCGAG-TTAAAGACCCGAGCCTCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGAGGCTCGGGTCTTTAACTCGAGTTAAAGACCCGAGCCTCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGAGGCTCGGGTCTTTAACTCGAGTTAAAGACCCGAGCCTCTTGG-3'

Other clones with same target sequence:

(none)